Dilated cardiomyopathy (DCM) is the most common cause of non-ischemic heart failure leading to heart transplantation. In the majority of cases the primary cause is unknown, giving rise to the term idiopathic DCM (IDCM). In the search of indicators and markers of this pathological process, the iCOR group proposes the low density lipoprotein (LDL) receptor-related protein 1 (LRP1) as a presumed biomarker for IDCM.
In our recently published work, PhD student Ana Gámez-Valero together with Dr Santiago Roura have described that LRP1 is not detectable in plasma-EVs from IDCM patients, but rather the soluble form of the protein can be detected in the systemic circulation showing increased levels in IDCM patients compared to age-matched controls.
Could this soluble part of the protein be exported in the membrane of EVs? Our preliminary contribution in this work showed that LRP1 soluble part cannot be detected by flow cytometry in platelet-free plasma derived EVs, neither in patients nor controls.
Nevertheless, this study opens new doors for further comprehensive analysis of the
cargo of these EVs released into the blood of IDCM patients and having systemic effects. We are currently studying the possibility of the presence of the cytoplasmic fragment of LRP1 inside the EVs. Proteomic analyses are on-going and we expect to have new results soon. Be alert and don’t miss a anything about it!
Roura S, Gálvez-Montón C, de Gonzalo-Calvo D, Valero AG, Gastelurrutia P, Revuelta-López E, Prat-Vidal C, Soler-Botija C, Llucià-Valldeperas A, Perea-Gil I, Iborra-Egea O, Borràs FE, Lupón J, Llorente-Cortés V, Bayes-Genis A. Extracellular vesicles do not contribute to higher circulating levels of soluble LRP1 in idiopathic dilated cardiomyopathy. J Cell Mol Med. 2017 May 29. doi: 10.1111/jcmm.13211